The fluorescent dye CFSE (CFDA-SE) is a novel dye that can fluorescently label living cells and can label living cells. The basic principle is as follows: CFSE can easily penetrate the cell membrane, covalently bind to intracellular proteins in living cells, and release green fluorescence after hydrolysis. In the process of cell division and proliferation, its fluorescence intensity decreases with the division of cells, and the labeled fluorescence can be evenly distributed to the two daughter cells, so its fluorescence intensity is half that of the parental cells. According to this characteristic, it Can be used to detect cell proliferation, cell cycle estimation and cell division. In addition, CFSE-labeled cells can be used for in vivo observations for up to several weeks. They are often used for live cell assays and for observation of long-term cellular activity using fluorescence electron microscopy. The fluorescent probe CFSE enters the cell and localizes to the cell membrane, cytoplasm and nucleus, and the fluorescent staining in the nucleus is the strongest, and it is confirmed that CFSE does not affect the cell proliferation ability. This method is simple to operate and does not use radioisotopes, and there is no safety hazard. Get the experimental data you want faster, more accurately and safely. advantage: use: other: Customized Led Road Stud,we offen said Solar Road Stud or solar road marker, is a kind of Road Stud, set along the road surface, used at night or in rainy and foggy weather, to indicate the direction of the road, composed of retroreflective material, shell, solar panel, LED, control device , A visual induction facility with active luminous and passive reflective properties, usually combined with markings. Customized Led Road Stud solar road stud lights,road stud reflectors,solar road stud lights,Garden solar Road Stud Shenzhen Wide Way Optoelectronics Co., Ltd. , https://www.wdmtrafficlight.com
CFSE: 5- or 6-(N-Succinimidyloxycarbonyl-3',6'-O,O'-diacetylfluorescein) 
1. In vivo labeling: It can track the proliferation and proliferation process of cells in the body.
2. Long time: The marked fluorescence can last for several weeks.
3. Safety: No radioisotopes are required.
4. High correlation: consistent with the results of the H3 incorporation method, it can replace the latter.
5. Specific Tracking: Can be used in conjunction with cell subpopulation specific antibodies.
6. Simple: Simple and reliable operation.
7. Stabilization: Fluorescence is not affected by the internal environment after entering the cell.
8. Objective results: no effect on cell physiological activity.
9. Affordable: Cost-effective and efficient.
1. Cell proliferation assay (especially lymphocyte proliferation assay).
2. Trace in the body.
3. Cellular fluorescent labeling.
4. Cytotoxicity test.
1. Detection equipment: fluorescence electron microscope, flow cytometer, CO2 incubator, pipette, etc.
2. Excitation wavelength: 500nm
3. Emission wavelength: 520nm
The reflective brightness of ordinary reflective road studs is only 300-400 MCD, while the luminous brightness of solar road studs can reach more than 2000 MCD, which is 6-7 times that of the former. High-intensity light can penetrate rain and fog at night, which is safe and effective. Provide directions for the driver.
Active light, dynamic warning
Solar road studs flicker at a certain frequency at night, and human vision is more sensitive to changes, so its dynamic warning effect is very strong. Active lighting can not only avoid the interference of rain and fog to the greatest extent, but also can break away from the dependence on car lights, and the visual distance is longer and the effect is better.
Solar road studs play a very important role in delineating road contours in rainy and foggy weather and at bends, inducing the driver's sight and eliminating potential safety hazards.